Question

9. Choose the odd one w.r.t. type of DNA ends
obtained after digestion with restriction enzyme.
(1) Eco RV
(2) Hind II
(3) Eco RI
(4) Sma!​

Answer 1

Sma! Is the odd one rest other are enzymes

Answer 2

Sma

Explanation:

The enzymes like Eco RV, Hind II and Eco RI are the enzymes that cut the DNA in the blunt ends. The simplest DNA end of the ds(DNA) molecule is known as blunt end.

The blunt end is not always desired in the biotechnology because ligase is used to join two molecules into one and the yield is lower with the blunt ends.

These enzymes are have disadvantage at the time of performing subcloning.

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