9. Use Figure 4-11 The Carbon Cycle on page 127 to answer the following. "Grandma Johnson died in a south Florida retirement community, after a long happy life. She was buried in Boca Raton, Florida. After a while, one of Grandma Johnson's carbon atoms ended up in a coyote in Southern California. • The coyote did not travel to Florida. • Grandma Johnson's body did not travel to California. • No humans'ate' Grandma Johnson. • Coyotes do not attack people (in this scenario) In a paragraph, describe the sources and processes in detail, about how Grandma Johnson's carbon ended up in the coyote.
Answers
Answer:
In the vast majority of cases, it is not necessary to remove the polyHis-Tag from recombinant proteins. The polyHis-tag is widely and preferably used for recombinant immunogen production because of its small size (0.84 KDa) and low immunogenicity. So, it doesn’t interfere with the immune response rised against the protein of interest. Although unusually, His-tag may affect the functionality of the protein. Then, take this into account if you will carried out functional assays and any problem arise. Anyway, my lab partners and I have produced many polyHis-tag recombinant antigens and they always resulted in both very good immunogenicity and functionality. Removing the tags can be a tedious labor and time compsuming, because depending on the proteins sequences, the protease treatments may result in protein degradation or losses. From my own experience, I usually recommend keeping the tag and not to remove it until interference evidences shows up during assays (which can not be solved by other strategy).
After a while, one of Grandma Johnson's carbon atoms ended up in a coyote in Southern California.
• The coyote did not travel to Florida.
• Grandma Johnson's body did not travel to California.
• No humans'ate' Grandma Johnson.