Question

A biochemist wishes to use histidine to buffer a solution at ph 8.57. Histidine has three ionizable groups, with pka values of 1.82, 6.0, and 9.17. She has 100 ml of a 0.1 m histidine solution at ph 5.4. What volume of 1.0 m naoh must she add to bring the ph to 8.57?

Answer 1

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Answer 2

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