A chromatographic technique which does not involve binding of the protein to a suppo
Answers
Chromatography is a versatile separation technique widely used to obtain pure compounds from mixtures. All chromatographic techniques depend on a stationary phase, usually a finely divided solid or coated solid, that a mobile phase, usually a gas or liquid, moves through.
Based on this approach three components form the basis of the chromatography technique.
Stationary phase: This phase is always composed of a “solid” phase or “a layer of a liquid adsorbed on the surface a solid support”.
Mobile phase: This phase is always composed of “liquid” or a “gaseous component.”
Separated molecules
Types of chromatography
Column chromatography
Ion-exchange chromatography
Gel-permeation (molecular sieve) chromatography
Affinity chromatography
Paper chromatography
Thin-layer chromatography
Gas chromatography
Dye-ligand chromatography
Hydrophobic interaction chromatography
Pseudoaffinity chromatography
High-pressure liquid chromatography (HPLC)
Explanation:
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