A lab activity: to observe the peels cell of onion
Answers
Aim - To observe nucleus in onion peel cell
Materials required - Onion peel , stain ( saffrain , methylene blue or red ink ) , slide , cover slip , filter paper and microscope
Procedure -
- Take a small fleshy portion of onion bulb and break it into pieces to obtain the thin translucent membrane
- Now keep the membrane on the slide and add a drop of saffrain or methylene blue for staining
- Cover the cover slip and leave it for few minutes
- Add few drops of water from one side of cover slip such that extra stain is washed away
- Use filter paper for soaking extra water
- Observe under microscope
Observation - A dark spot can be observed towards the periphery which is nucleus . Thick boundry of cell called as cell wall is also visible
Inference - Onion peel consists of dense round body called nucleus
Precautions - Tap the cover slip to remove air bubbles
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To observe the cells of onion peel
Onion peel, slide, glycerine,
microscope
➜Take an onion peel
➜Now place it on slide
➜Put a drop of water and then a drop of glycerine on it.
➜Gently cover it with a cover slip.
➜Observe it under the microscope.
Rectangular shaped cells are observed the microscope.
Onion peel cells are observed under the microscope.