Question

A mixture contains three similarly sized peptides P, Q and R. The peptide P is positively
charged, Q is weakly negative and R is strongly negative. If this mixture is passed through
an ion-exchange chromatography column containing an anionic resin, their order of elution
will be
(A) P, Q, R (B) R, Q, P
(C) Q, R, P (D) P, Q and R elute together

Answer 1

$\huge{Hello Friend}$

The answer of u r question is..✌️✌️

Option.B

Thank you..⭐️⭐️

Answer 2

Answer:

If this mixture is passed through  an ion-exchange chromatography column containing an anionic resin, their order of elution  will be (A) P, Q, R

Positively charged most proteins will be eluted first and most negatively charged proteins will be the last.

Explanation:

Proteins will elute in order of their net charge. In case of anion exchange column net positively charged protein would elute prior to the net negatively charged protein. Ion exchange chromatography resin binds with the proteins from the solution with the help of ionic interaction. The order of protein binding with the resin depends on the isoelectric point of the protein and total Ki value of all charged amino acids in the protein. At buffer pH level higher isoelectric point of proteins will result into negatively charged protein while lower isoelectric point of the protein will result into the positively charged proteins.