Biology, asked by Amil25091, 1 year ago

A novel compound was identified that binds to thymine. When two bacteria strains that had different sizes of dna was stained (colored), it was observed that the amount of staining was same in both the cells. What might be the reason?

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Answered by shubham000020
0

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possibilities. They grew E. coli cells in a medium containing the heavy isotope of nitrogen (15N) rather than the normal light (14N) form. This isotope was inserted into the nitrogen bases, which then were incorporated into newly synthesized DNA strands. After many cell divisions in 15N, the DNA of the cells were well labeled with the heavy isotope. The cells were then removed from the 15N medium and put into a 14N medium; after one and two cell divisions, samples were taken. DNA was extracted from the cells in each of these samples and put into a solution of cesium chloride (CsCl) in an ultracentrifuge.❤️❤️❤️❤️

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