Question

A PCR machine can rise temperature upto
100 °C but after that it is not able to lower the temperature below 70 °C automatically. which step
of PCR will be hampered first in this faulty machine?explain why?​

Answer 1

Explanation:

step -1 : Denaturation. As in DNA replication , the two strands in the DNA double helix need to be separated. ...

step -2 : Annealing. primers bind to the target DNA sequences and initiate polymerisation. ...

step -3 : Extencsion . new strands of DNA are made using the original strands as templates

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