A PCR machine can rise temperature upto 100°C but after it is not able to lower the temperature below 70°C automatically. Which step of PCR machine will be hampered first in this faulty machine? Explain ?
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Step 1: Denaturation. As in DNA replication, the two strands in the DNA double helix need to be separated. ...
Step 2: Annealing. Primers bind to the target DNA sequences and initiate polymerisation. ...
Step 3: Extension. New strands of DNA are made using the original strands as templates.
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ur answer is primer design.
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