Question

A plasmid dna and a linear dna (both of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one dna band while, linear dna shows two fragments. Explain.

Answer 1

linear DNA have a site for restrictions endonucleus where the restriction endinucleus work on it and divide it in two parts and plasmid DNA have no site for restriction endonucleus so it has single band