Question

A protein with molecular weight of 20000 Dalton contains five and four charges. At

what M/Z are protein ions detected by mass spectrometer?​

Answer 1

Answer:

protein MW determination by MS

(NOT= identification)

-for MW determination the protein needs to be

in solution without salts and detergents

-usually proteins are first purified with

RP chromatography before MW measurement

2

-MALDI TOF MS, linear mode

-accuracy is not as good with ESI MS