Question

A rDNA is inserted in the coding sequence of an enzyme and which in activates the gene give the term for that.
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Answer 1

Explanation:

ANSWER :-

This will result in the activation of enzyme and the recombinant colonies do not produce any colour.

DETAILS:-

Blue white screening or insertional inactivation is a kind of method of selection and screening of transformed cells. In this method, the recombinants are differentiated from non recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. The rDNA or recombinant DNA is inserted within the coding sequence of the enzyme galactosidase. This results in inactivation of the enzyme. In the presence of chromogenic substrate, blue coloured colonies are produced if the plasmid in the bacteria doesn't have the insert i.e. those are non recombinant. The presence of inserts in the bacteria (recombinant) result in the inactivation of galactosidase enzyme due to which colourless colonies are produced.

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@MissSharma1⚡

Answer 2

Answer:

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Explanation:

In this, a recombinant DNA is inserted within the coding sequence of an enzyme, p-galactosidase. This results into inactivation of the enzyme, which is referred to as insertional inactivation. The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert