Question

A tube with water how to say if it is having protein

Answer 1

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Here is the answer
normally protein induction is done with an OD600 between 0.5 - 1.0. The cells should be in the logarithmic phase. Induction time varies between 2h up to over night or even loger. Normally 3h are a good starting point. Grow your cells until they have reached OD ~0.7 and induce with 1mM IPTG. Take an aliquot for SDS-PAGE after 1h, 2h, 3h and 4hrs and analysis. This will give you a good starting point for your protein synthesis. When starting testing clones it is always good to start with a fresh strike out or fresh transformation and test several clones. Sometime you can see differences between your clones even when all shoul be identical

Answer 2

Answer:

normally protein induction is done with an OD600 between 0.5 - 1.0. The cells should be in the logarithmic phase. Induction time varies between 2h up to over night or even loger. Normally 3h are a good starting point. Grow your cells until they have reached OD ~0.7 and induce with 1mM IPTG. Take an aliquot for SDS-PAGE after 1h, 2h, 3h and 4hrs and analysis. This will give you a good starting point for your protein synthesis. When starting testing clones it is always good to start with a fresh strike out or fresh transformation and test several clones. Sometime you can see differences between your clones even when all shoul be identical

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