Question

A unique rna species from maturing mouse spermatozoa

Answer 1

Answer:

STUDIES on ejaculated spermatozoa have shown that RNA is either absent or present only in trace amounts1-4. Work on RNA synthesis during spermatogenesis, however, suggested that RNA might be present in maturing spermatozoa. Although histochemical techniques disclosed little cytoplasmic RNA after the second meiotic division5, Monesi's autoradiographic studies on mouse testes have demonstrated a small burst of post-meiotic nuclear RNA synthesis that declined upon spermatozoan maturation6. Moore has also shown active RNA polymerase in early spermatids7. Although RNA has not been quantitatively detected in ejaculated spermatozoa, incorporation of radioactive RNA precursors has been demonstrated8 and we have extended these findings. Our studies on RNA metabolism in maturing mouse spermatozoa (from the epididymis and the ductus deferens) reveal the presence of larger amounts of RNA than previously reported for ejaculated spermatozoa. We have not investigated the RNA content of the ejaculated spermatozoa

Answer 2

Answer:

Explanation:

STUDIES on ejaculated spermatozoa have shown that RNA is either absent or present only in trace amounts 1–4. Work on RNA synthesis during spermatogenesis, however, suggested that RNA might be present in maturing spermatozoa. Although histochemical techniques disclosed little cytoplasmic RNA after the second meiotic division 5, Monesi's autoradiographic studies on mouse testes have demonstrated a small burst of post-meiotic nuclear RNA synthesis that declined upon spermatozoan maturation 6. Moore has also shown active RNA polymerase in early spermatids 7. Although RNA has not been quantitatively detected in ejaculated spermatozoa, incorporation of radioactive RNA precursors has been demonstrated 8 and we have extended these findings. Our studies on RNA metabolism in maturing mouse spermatozoa (from the epididymis and the ductus deferens) reveal the presence of larger amounts of RNA than previously reported for ejaculated spermatozoa. We have not investigated the RNA content of the ejaculated spermatozoa.

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