activity to show chemotropism
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Chemotropism assay showing pollen tube reorientation over time ( A and B ) and quanti fi cation method ( C ). Wells containing 1.5 g ͞ l SPs ( A ) or water ( B ) were prepared 40 min before application at 0 h of pregerminated pollen with their tubes manually oriented away from the well. At 5 h, some pollen tubes in the control were out of the fi eld (data not shown). A single reorienting pollen tube (indicated with arrows) monitored over time is enlarged ( Inset ). ( C ) Reorientation of a pollen tube by 90 ° or more was counted as positive chemotropism. (Bar ϭ 2 mm.)
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Chemotropism assay showing pollen tube reorientation over time ( A and B ) and quanti fi cation method ( C ). Wells containing 1.5 g ͞ l SPs ( A ) or water ( B ) were prepared 40 min before application at 0 h of pregerminated pollen with their tubes manually oriented away from the well. At 5 h, some pollen tubes in the control were out of the fi eld (data not shown). A single reorienting pollen tube (indicated with arrows) monitored over time is enlarged ( Inset ). ( C ) Reorientation of a pollen tube by 90 ° or more was counted as positive chemotropism. (Bar ϭ 2 mm.)
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