Question

Advantages of calcium phosphate dna transfection method

Answer 1

Answer:

The principle of calcium phosphate co-precipitation involves mixing DNA with calcium chloride in a buffered saline/phosphate solution to generate a calcium-phosphate–DNA co-precipitate, which is then dispersed onto cultured cells. Calcium phosphate facilitates the binding of the condensed DNA in the co-precipitate to the cell surface, and the DNA enters the cell by endocytosis. Aeration of the phosphate buffer while adding the DNA-calcium chloride solution helps to ensure that the precipitate that forms is as fine as possible, which is important because clumped DNA will not adhere to or enter the cell as efficiently.

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