Question

Advantages of running in gradient gel electrophoresis

Answer 1

There are three main advantages of gradient gels over linear gels: 1.The advancing edge of the migrating protein zone is retarded more than the trailing edge, thus resulting in a sharpening of the protein bands.2.The gradient in pore size increases the range of molecular weights that can be fractionated in a single gel run.3.Proteins with close molecular weight values are more likely to separate in a gradient gel than a linear gel.

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