Question

After protein precipitation, pellet should be dissolved in?

Answer 1

$\huge \blue {hello \: buddy}$
$\small \bold \red { your \: answer}$

I would suggest you to try to vortex your pellet with sample buffer first before putting it onto the hot plate and add more sample buffer to dissolve it. Normally when I encounter TCA precipitation, I will load 60uL of 1X sample buffer to the pellet in a eppendorf and have no trouble dissolving with various recombinant protein(whether soluble or inclusion body)

As to your second point of view, I think its fine but you need to make sure the protein as a high enough concentration in the SDS-PAGE for western or do a TCA precipitation after sonication. As for me, my protein's signal is way to high on Western after sonication then TCA precipitation.
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Answer 2

HELLO BUDDY ❤

Answer:

I would suggest you to try to vortex your pellet with sample buffer first before putting it onto the hot plate and add more sample buffer to dissolve it. Normally when I encounter TCA precipitation, I will load 60uL of 1X sample buffer to the pellet in a eppendorf and have no trouble dissolving with various recombinant protein(whether soluble or inclusion body)

As to your second point of view, I think its fine but you need to make sure the protein as a high enough concentration in the SDS-PAGE for western or do a TCA precipitation after sonication. As for me, my protein's signal is way to high on Western after sonication then TCA precipitation.

I hope this helps. It's my first time answering question. So if anything is unclear, please do let me know. Thanks.