an amino acid has three ionizable groups with pka of 2.0,10.5,and 3.8. what is the pI of amino acid
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pI of the amino acid which has three ionisable groups with pKa of 2.0, 10.5,and 3.8 is equal to 3.9
- pI of an amino acid is defined as the pH at which the amino acid is at the isoelectronic point.
- The isoelectric point is a condition in which the net chrge on the amino acid is equal to zero.
- For an amino acid containing two ionizable groups the pI is equal to the mean of the pKa of the two groups.
- In case of three ionizable groups, the pI is equal to the average of the pKa of the two groups which are either both having pKa greater than 7 or both having pKa less than 7.
- In this case pI = (2.0+3.8)/2 = 3.9
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