An analysis of bacteria separation and filtration from blood sample utilizing colander/strainer geometrical network in biomems using passive methods- dynamic simulation approach
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A species-specific, quantitative PCR assay for the Altered Schaedler Flora is described.
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This SYBR green-based assay can be used with both fecal and cecal samples.
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Primers target the hypervariable regions of the 16S rDNA of each ASF taxon to enhance assay specificity.
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The presence of lowly abundant ASF taxa can be determined via a nested PCR assay.
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This qPCR assay provided robust results under normal physiological conditions and when the community was subject to perturbation.
•
This SYBR green-based assay can be used with both fecal and cecal samples.
•
Primers target the hypervariable regions of the 16S rDNA of each ASF taxon to enhance assay specificity.
•
The presence of lowly abundant ASF taxa can be determined via a nested PCR assay.
•
This qPCR assay provided robust results under normal physiological conditions and when the community was subject to perturbation.
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