An environment free from the growth of microbes
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In order to grow successfully, microorganisms must have a supply of water as well as numerous other substances including mineral elements, growth factors, and gas, such as oxygen. Virtually all chemical substances in microorganisms contain carbon in some form, whether they be proteins, fats, carbohydrates, or lipids.
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Every species of microbe has evolved adaptations that enable them to live under specific environmental conditions, which we refer to as their niche. These include oxygen (O2) concentration, temperature, pH, light intensity, radiation, pressure, solute concentration (osmolarity) and water activity.
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