Question

An enzyme catalyzes the reaction A  B. The initial rate of the reaction was measured as a function of the concentration of A. The following data were obtained:
[A], micromolar V0, nmoles/min
0.05 0.08
0.1 0.16
0.5 0.79
1 1.6
5 7.3
10 13
50 40
100 53
500 73
1,000 76
5,000 79
10,000 80
20,000 80
a) What is the Km of the enzyme for the substrate A?
b) What is the value of V0 when [A] = 43?
The above data was plotted as 1/ V0 vs. 1/[A], and a straight line was obtained.
c) What is the value of the y-intercept of the line?
d) What is the value of the x-intercept of the line?

Answer 1

Explanation:

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