Biology, asked by Abhi8725, 9 days ago

An enzyme of mol. wt.= 26,000 and pI=5.5 is contaminated with a protein of similar molecular weight but with pI=7.2, and another protein of molecular weight= 12,000 and pI=5.4. Suggest a purification strategy.

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Answered by Anonymous

Answer:

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Explanation:

REFLECT AND APPLY You wish to separate and purify enzyme A from contaminating enzymes and . Enzyme is found in the matrix of the mitochondria. Enzyme is embedded in the mitochondrial membrane, and enzyme is found in the peroxisome. Enzymes A and B have molecular weights of 60,000 Da. Enzyme C has a molecular weight of 100,000 Da. Enzyme A has a pI of 6.5 Enzymes B and C have pI values of Design an experiment to separate enzyme A from the other two enzymes.

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An enzyme of mol. wt.= 26,000 and pI=5.5 is contaminated with a protein of similar molecular weight but with pI=7.2, and another protein of molecular weight= 12,000 and pI=5.4. Suggest a purification strategy.​

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An enzyme of mol. wt.= 26,000 and pI=5.5 is contaminated with a protein of similar molecular weight but with pI=7.2, and another protein of molecular weight= 12,000 and pI=5.4. Suggest a purification strategy.