Question

An eukaryotic cell non functional tba polymerase I may not form

Answer 1

1.DNA polymerase is the enzyme that catalyse the synthesis of DNA and are of two types: template dependent and template independent.

2.Template dependent DNA polymerases are further of two types, DNA dependent DNA polymerases and RNA dependent DNA polymerases.

3.DNA dependent DNA polymerase which is simply called as DNA polymerase synthesises a new strand of DNA on a DNA template

4.whereas RNA dependent DNA polymerase which is also called as reverse transcriptase, synthesises a new strand of DNA on a RNA template.

5.DNA polymerase pairs the complementary nucleotides with the template strand which are then joined by DNA ligase.

6.Hence, if mutation occurs in a gene encoding for DNA polymerase, then DNA polymerase will not work and DNA synthesis will not occur.

7.The main function of DNA polymerase is to synthesize DNA form deoxyribonucleicecotides, the building blocks of DNA. The DNA copies are created by the pairing of nucleotides to bases present on each strand of the original DNA molecule.

8.Eukaryotic cells contain 5 different DNA polymerases: α, β, γ, δ, and ε.

9.Eukaryotic DNA polymerase β is most similar to E. coli DNA Pol I because its main function is associated with DNA repair, rather than replication. DNA polymerase β is mainly used in base-excision repair and nucleotide-excision repair.

10. Polymerase γ is located in mitochondria and is responsible for replication of mitochondrial DNA. The other four enzymes are located in the nucleus and are therefore candidates for involvement in nuclear DNA replication.

Answer 2

Answer:

An eukaryotic cell non functional DNA polymerase I will disturb the process of DNA replication.

Explanation:

• Each parental strand of DNA acts as a template for the synthesis of a new complementary daughter strand during the semiconservative process of replication. DNA polymerase, which is a key enzyme in this process, catalyzes the joining of deoxyribonucleoside 5′-triphosphates (dNTPs) to create the expanding DNA chain. However, compared to a single enzyme activity, DNA replication is significantly more complex.
• The primary enzyme in charge of E. coli DNA replication is not the first DNA polymerase ever discovered, now known as DNA polymerase I. Instead, it is now understood that DNA replication and repair are carried out by a variety of DNA polymerases found in both prokaryotic and eukaryotic cells.

#SPJ2