An Hfr strain with genotype maghu letop' and that transiens the metgene very late was mated with a laths) recipient. The hus(13) mutation intro dooss a requirement for histidine s 42°C. After mating for several hours, the mi ture was duoted and plated on minimal media with four different supplements. The plates were incubated at 12°C. The supplements in the plates and the number of colonies per plate are the following His Thp 250 His Leu 50 Leu Thy 500 His 10
a. What is the purpose of the met mutation in the Hfr strain in this experiment?
b. Which genes entered first second and third
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