Analysis of soaps to determine the amount of glycerine
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A simple and rapid procedure is described for the isolation, silylation and capillary gas chromatographic quantitation of the free glycerin content in soap. Free glycerin is determined by mixing the sample with dimethylformamide (DMF), filtering the mixture, silylating an aliquot withbis-trimethylsilyltrifluoroacetamide (BSTFA), and quantitating by capillary GC using flame ionization detection. Silyl derivatization and capillary gas chromatography provide for a quick and easy analysis which allows straightforward automated gas chromatographic analysis instead of the more tedious traditional periodate methods. This procedure also provides reliable quantitation for glycerin levels in soap lower than those measurable with the standard methods