Question

Analytical methods like turbidity methods in cells

Answer 1

urbidity results in a decrease of intensity of the light beam that passes though a turbid solution due to light scattering, reflectance, and absorption. Measurement of this decreased intensity of light is measured in turbidimetric assays. However, in nephelometry, light scattering is measured. In common nephelometry, scattered light is measured at a right angle to the scattered light. Antigen–antibody reactions may cause turbidity, and either turbidimetry or nephelometry can be used in an immunoassay for quantification of an analyte. Therefore, both nephelometry and turbidimetry are spectroscopic techniques. Although nephelometry can be used for analysis of small molecules, it is more commonly used for analysis of relatively big molecules such as immunoglobulin, rheumatoid factor, etc.