Answer of a vector can accept an insert of 20kb size and the recombinant vector can be replicated in
e.Coli.In order to make complete gene library of
e.Coli by this vector,minimum number of bacterial colonies which must be present should not be less than
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Successful development of recombinant DNA methods will provide tools that ... Step 2: This cloning vector–insert DNA construct is transferred into and maintained within a host cell.
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