Question

any molecule comprises of 546 nucleotides how many cytosine nucleotides would be present in it if the number of any nucleotides is 96​

Answer 1

Detection of nucleotide

Detection of nucleotide modifications is based on either of three basic principles. For two of these, that is, separation according to physicochemical properties and differential enzymatic turnover, we refer to other reviews [4, 7, 9–12] and will mention such methods only when they are combined with the use of chemicals that specifically react with modified nucleotides. Here, we focus on the third principle, which is differential chemical reactivity. To set the stage, we will outline the known reactivity of the four major ribonucleotides in this paragraph. In principle, any reagent that reacts with nucleotides may be considered for chemical recognition of nucleotide modification, provided that conditions can be determined, where its reactivity significantly differs between a given major nucleotide and its modified counterpart. In the best case, conditions would be optimized to the point of exclusive reaction with either the standard or the modification. From a practical point of view, it is helpful to distinguish reagents within a narrowly defined window of experimental conditions from those which reliably and completely discriminate nucleotides over a wide plateau of experimental conditions. The former methods are evidently more difficult to newly establish in a laboratory, despite the emergence of numerous publications covering detailed protocols, and should by tackled only by experienced RNA scientists. However, even for the less complicated reactions, one should be conscious that deviations in temperature, pH, salt, incubation time, or reagent concentration may result in “leaving” the plateau and thus result in suboptimal discrimination. Similar considerations apply to the detection of the chemical species resulting for treatment with the discriminating reagent. Most often, the ease and turnaround time of detection will determine if a given reagent finds widespread use in the RNA community.

The above considerations apply to reagents of various types alike, which we have grouped into electrophiles, nucleophiles, and oxidizing, and reducing agents.

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