Question

Application of nesslers reagent in biology

Answer 1

$\huge\orange{Hello there!}$ An aques solution of potassium iodide, mercuric chloride, and potassium hydroxide use as a test for the presence of ammonia
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Answer 2

The instability of conventional Nessler reagents is shown to be the cause of inaccuracy when they are used to estimate ammonia in solution. A critical investigation of the Nessler reaction has resulted in the formulation of a satisfactory, aqueous reagent. Use of this reagent along with gum arabic, which protects the ammonia‐colour‐complex from flocculation, has permitted the problem of interference to be clarified and by incorporating a method for controlling interference a procedure has been worked out for directly nesslerising Kjeldahl digests. When applied to the determination of the nitrogen content of plant material, the procedure is comparable in accuracy and precision to that of a distillation‐titration method. The reagent is sensitive to 5 μg. of ammoniacal nitrogen in solution and the precision of the method is about 1·4%.