Question

At what [S] is the velocity (Vo) of an enzyme-catalyzed reaction is 25% of Vmax?​

Answer 1

Answer:

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here is your answer

Vmax​ or maximum velocity of enzyme catalyzed reaction is a fundamental characteristic of enzyme. It depends on the number of active sites in enzymes and can be directly correlated with the substrate concentration at lower substrate concentration. In other words, at low substrate concentration, the Vmax​ of a reaction will increase with the concentration of substrate molecules till all the available active sites are saturated with the substrate molecules. However, it is difficult to exactly calculate Vmax​. Hence, Vmax​ is not a measure for the purpose of enzyme assay. It is usually deduced graphically. The reaction rate is measured at different substrate concentration and a graph is drawn. From the graph, Vmax​ and optimum substrate concentration can be deduced