Automated DNA sequencing is an improvement of Sanger's method where
a.
Flourescently labelled ddNTPs are used for chain termination method
b.
Flourescently labelled dNTPs are used for chain termination method
c.
ddNTPs are used for chain termination
d.
PCR is used for making sequencing templates
Answers
Explanation:
a).Automatic sequencing still uses the chain termination method of sequencing, but the dideoxynucleotides are each labeled with a different dye. ... Each dye is automatically recorded by a sequencing machine containing a fluorescent detection system and assembled into complete sequence using a computer.
b).The Sanger Method is used to amplify a target segment of DNA, so that the DNA sequence can be determined precisely. The incorporation of ddNTPs in the reaction valves are simply used to terminate the synthesis of a growing DNA strand, resulting in partially replicated DNA fragments
c).Dideoxynucleotides are chain-elongating inhibitors of DNA polymerase, used in the Sanger method for DNA sequencing. They are also known as 2',3' because both the 2' and 3' positions on the ribose lack hydroxyl groups, and are abbreviated as ddNTPs (ddGTP, ddATP, ddTTP and ddCTP).
d).DNA Sequence For Chain Termination PCR
The DNA sequence of interest is used as a template for a special type of PCR called chain-termination PCR. ... The result of chain-termination PCR is millions to billions of oligonucleotide copies of the DNA sequence of interest, terminated at a random lengths (n) by 5'-ddNTPs.