Question

Based on the genetic transmission in plants..please answer the question​

Answer 1

INDIRECT OR VECTOR-MEDIATED GENE TRANSFER:

• Gene transfer is mediated with the help of a plasmid vector known as indirect or vector-mediated gene transfer.
• Among various vectors used for plant transformation, Ti - plasmid from Agrobacterium tumefaciens has been used extensively.
• This bacterium has a large sized plasmid, known as Ti - plasmid and a portion of it referred as T-DNA is transferred to plant genome in the infected cells and cause plant tumors.
• Since the bacterium has the natural ability to transfer T-DNA region of its plasmid into plant genome, upon infection of cells at the wound site,it is also known as the natural genetic engineer of plants.
• The foreign gene and plant selection marker gene, usually an antibiotic gene like npt II which confers resistance to antibiotic kanamycin are cloned in the T-DNA region of Ti - plasmid in place of unwanted DNA sequences.