Can anybody help me for giving the biology experiment of onion peel?
With its diagram?
Answers
Hey ❤
Experimental Preparation of Temporary Mounts of an Onion Peel !
Experiment:
Objective:
To prepare a stained temporary mount of an onion peel and to record observations and draw labeled diagrams.
Apparatus and materials required:
An onion, glass slide, watch glass, coverslip, forceps, needles, brush, blade, filter paper, safranin, glycerine, dropper, water, and a compound microscope.
Theory:
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All living organisms are made up of cells. The shape, size and the number of these units vary in organisms. The three major components of a cell are the cell membrane, cytoplasm and nucleus. In a plant cell, a cell wall surrounds the cell membrane.
Procedure:
1. Take an onion and remove its outermost peel.
2. Now cut a small part from an inner scale leaf with the help of a blade.
3. Separate a thin, transparent peel from the convex surface of the scale leaf with the help of forceps.
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4. Keep this peel in a watch glass containing water?
5. Add two drops of safranin stain in the watch glass to stain the peel.
6. Take a clean slide and put a drop of glycerine in the centre of the slide.
7. With the help of a brush and needle transfer the peel on the slide. Glycerine prevents the peel from drying up.
8. Carefully cover it with a coverslip and avoid any air bubble from entering interring the coverslip.
9. Remove any excessive glycerine with a filter paper.
10. Observe the prepared mount of the peel under the low and high magnification of a compound microscope.
Observations:
A large number of rectangular cells are visible. These cells lie close to each other with intercellular spaces between them. These cells are surrounded by distinct cell walls. These cells have a dark stained nucleus and a large vacuole in the centre.
(a)-(b) Methods of Separating an Onion Peel (c) Structure of Onion Cells as Seen under a Microscope (450 x)
Precautions:
1. Overstaining and understaining should be avoided.
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2. Folding of the peel should be avoided.
3. Clean and dry glass slide and coverslip should be used.
4. Coverslip should be put carefully avoiding any air bubbles.
take a onion and peel off its skin and then remove its membrane apply some pink or eney food colour in water and put it on the membrane put this arrangement on a slide and put a cover slit and view it under microscope
