can primers be renewed in PCR tests
Answers
Answer:
These sequences are intended to be used for the purposes of respiratory virus surveillance and research. The recipient agrees to use them in compliance with all applicable laws and regulations. Every effort has been made to assure the accuracy of the sequences, but CDC cannot provide any warranty regarding their accuracy. The recipient can acknowledge the source of sequences in any oral presentations or written publications concerning the research project by referring to the Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
2019-Novel Coronavirus (2019-nCoV) Real-time rRT-PCR Panel Primers and Probes
Name Description Oligonucleotide Sequence (5’>3’) Label1 Final
Conc.
2019-nCoV_N1-F 2019-nCoV_N1 Forward Primer GAC CCC AAA ATC AGC GAA AT None 500nM
2019-nCoV_N1-R 2019-nCoV_N1 Reverse Primer TCT GGT TAC TGC CAG TTG AAT CTG None 500nM
2019-nCoV_N1-P 2019-nCoV_N1 Probe FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1 FAM, BHQ-1 125nM
2019-nCoV_N1-P 2019-nCoV_N1 Probe FAM-ACC CCG CAT /ZEN/ TAC GTT TGG TGG ACC-3IABkFQ FAM, ZEN, 3IABkFQ 125nM
2019-nCoV_N2-F 2019-nCoV_N2 Forward Primer TTA CAA ACA TTG GCC GCA AA None 500nM
2019-nCoV_N2-R 2019-nCoV_N2 Reverse Primer GCG CGA CAT TCC GAA GAA None 500nM
2019-nCoV_N2-P 2019-nCoV_N2 Probe FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1 FAM, BHQ-1 125nM
2019-nCoV_N2-P 2019-nCoV_N2 Probe FAM-ACA ATT TGC /ZEN/ CCC CAG CGC TTC AG-3IABkF FAM, ZEN, 3IABkFQ 125nM
RP-F RNAse P Forward Primer AGA TTT GGA CCT GCG AGC G None 500nM
RP-R RNAse P Reverse Primer GAG CGG CTG TCT CCA CAA GT None 500nM
RP-P RNAse P
Probe FAM – TTC TGA CCT GAA GGC TCT GCG CG – BHQ-1 FAM, BHQ-1 125nM
RP-P RNAse P
Probe FAM-TTC TGA CCT /ZEN/ GAA GGC TCT GCG CG-3IABk
Explanation:
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Answer:
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Explanation:
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