Question

Cell disruption method for intracellular products physical and chemical methods

Answer 1

Disruption methods include non-mechanical and mechanical methods. Non-mechanical methods are further divided into physical, chemical and enzymatic disruption methods. In mechanical cell disruption, the cell is physically broken down with for example high-pressure homogenization or freezing and grinding the cells.

Answer 2

addition to physical and mechanicalmethods, several chemical methods forcell disruption exist. ... The mechanisms of actions are multiple, but the most widely used methods act by destroying the cellwall by enzymes, osmotic pressure, or by interfering or precipitating cell wall proteins.