Question

Cell separation techniques and centrifugation

Answer 1

Isolation of one or multiple cell types from a heterogeneous population is an integral part of modern biological research and routine clinical diagnosis and treatment. Purification of specific cells is essential for basic cell biology research, cellular enumeration in certain pathologies and cell based regenerative therapies. The main principle of separating any cell type from a population is to utilize one or more properties that are unique to that cell type. The most widely used cell isolation and separation techniques can be broadly classified as based on adherence, morphology (density/size) and antibody binding. The high precision single cell isolation methods are usually based on one or more of these properties while newer techniques incorporating microfluidics make use of some additional cellular characteristics.


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