Cellular components can be physically separated from each other by
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Proteins and other cell components must be separated for their individual properties to be studied. Different separation techniques are based upon different parameters, such as size, charge, and affinity, and can often involve multiple parameters.
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Most fractionation procedures begin with differential centrifugation at increasingly higher speeds (Figure 5-23), also called differential-velocity centrifugation. The different sedimentation rates of various cellular components make it possible to separate them partially by centrifugation.
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