characterization of protein by dialysis?
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Dialysis Methods for Protein Research
Dialysis Methods for Protein ResearchSee Navigation
Dialysis Methods for Protein ResearchSee NavigationPierce Protein Methods
Dialysis Methods for Protein ResearchSee NavigationPierce Protein MethodsIn working with proteins and nucleic acids, it is often necessary to eliminate small molecular weight substances such as reducing agents [dithiothreitol (DTT), 2-mercaptoethanol (BME)], non-reacted crosslinking or labeling reagents (sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (sulfo-SMCC), biotin) or preservatives (sodium azide, thimerosol) that might interfere with a subsequent step in the experimental procedure. Similarly, it is often desirable to exchange the protein sample into a different buffer system for downstream application such as electrophoresis, ion exchange or affinity chromatography. Dialysis is one method for accomplishing both contaminant removal and buffer exchange for macromolecular samples such as proteins.