Question

Chemical method of yeast alanyl t rna gene synthesis

Answer 1

Synthesis of oligonucleotid.es. Fifteen oligonucleotides ranging from pentanucleotide (5 bases) to an icosanucleotide (20 bases) were synthesized. Synthesis was conducted through condensation between hydroxyl group at the 3' position of one nucleotide and phosphate group at 5' position of the second nucleotide. In order to bring about condensation, all other functional groups, nottaking part in condensation were protected using specific protective groups. After protecting the groups, reaction between a nucleotide with protected 5' end and another nucleotide with protected 3' end proceed. Subsequently condensation was also brought about between di-, tri- or tetranucleotides.

Answer 2

Explanation:

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