Question

Cloning can be done in vitro, via ______
a. polymerase chain reaction.
b. gel electrophoresis.
c. transposons
d. lambda phage.

Answer 1

Polymerase chain reaction

Answer 2

Hii Madani,
First of all you need to know what in vivo & in vitro means -
- In vivo - technique performed inside the body of living thing.
- In vitro - technique performed outside the body of living thing.


◆ Answer -
Cloning can be done in vitro via *polymerase chain reaction*.

◆ Extra information -
- In vivo cloning is done inside the bacterial body. It involves DNA polymerase (taq polymerase) enzymes.
- In vitro cloning is done by polymerase chain reaction. It involves ligases, endonucleases.


Hope this is helpful..