Compare the role of enzymes dna polymerase and dna ligase in replication fork of dna
Answers
Answer:The role of DNA polymerases is to accurately and efficiently replicate the genome in order to ensure the maintenance of the genetic information and its faithful transmission through generations...in 5' to 3' direction by adding nucleotides which is complimentary to original strand....
The role of Dna Ligase is to join Okazaki fragmentsformed during replication, close repairs and seal recombination fragments by phosphodiester bond. This is the enzyme that is responsible for the breaking down of phosphate-sugar backbone of DNA....
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D.N.A replication is a semiconservative process in which each parental strand serves as a template for the synthesis of a new complementary daughter strand. The central enzyme involved is DNA polymerase, which catalyzes the joining of deoxyribonucleoside 5′-triphosphates (dNTPs) to form the growing DNA chain. However, DNA replication is much more complex than a single enzymatic reaction. Other proteins are involved, and proofreading mechanisms are required to ensure that the accuracy of replication is compatible with the low frequency of errors that is needed for cell reproduction. Additional proteins and specific DNA sequences are also needed both to initiate replication and to copy the ends of eukaryotic chromosomes.