Question

compare TLC and paper chromatography techniques

Answer 1

Explanation:

The basic difference between thin layer chromatography(TLC) and paper chromatography(PC) is that , while the stationary phase in PC is paper, the stationary phase in TLC is a thin layer of an inert substance supported on a flat, unreactive surface. ... Paper chromatography is performed using paper.

Answer 2

Answer:

Explanation:

The key differences between paper and thin layer chromatography are as follows:

1. The principle behind the paper chromatography is based on “Partition”. The principle behind the thin layer chromatography is based on “Adsorption”.

2. Paper chromatography requires less preparation whereas thin layer chromatography requires more preparation time.

3. The stationary phase of paper chromatography is the water trapped in the cellulose filter paper. The stationary phase of the thin layer chromatography is the glass plates coated with silica gel.

4. Thin-layer chromatography makes the use of silica gel whereas paper chromatography does not.

5. Corrosive reagents can be used in thin layer chromatography whereas paper chromatography does not, as the corrosive agents can destroy the paper.

6.The different spots can be seen under the UV-lamp in the thin layer chromatography whereas in paper chromatography cannot see under the UV-light.

7.Paper chromatography requires less time for particle separation whereas thin layer chromatography requires more time.