Confirmatory test for methyl ester with thin layer chromatography
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Thin-layer chromatography of glycolipids is performed on Silica gel plates, mainly from Merck (Germany). The application of Kieselgel 60F254 plates of the same company with preapplied fluorochrome makes it possible to determine the location of stains under UV illumination. We have used this method to obtain purified preparations; the stains were scraped off and eluted from Silica gel with methanol. Table 2.2 shows some of the solvent systems used for the thin-layer chromatography of glycolipids.
The chromatograms of glycolipids are stained for analytical purposes by moistening or spraying with 5% sulfuric acid solution in ethanol and heating to ~200°C. The following detection reagent is also used: 10.5 ml α-naphthol (15% in ethanol), 6.5 ml sulfuric acid, 40.5 ml ethanol, and 4 ml of distilled water. MEL was detected on the chromatograms using 0.2% anthrone in 75% sulfuric acid; the chromatogram was sprayed with the solution and heated to 110°C
The chromatograms of glycolipids are stained for analytical purposes by moistening or spraying with 5% sulfuric acid solution in ethanol and heating to ~200°C. The following detection reagent is also used: 10.5 ml α-naphthol (15% in ethanol), 6.5 ml sulfuric acid, 40.5 ml ethanol, and 4 ml of distilled water. MEL was detected on the chromatograms using 0.2% anthrone in 75% sulfuric acid; the chromatogram was sprayed with the solution and heated to 110°C
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