Criteria for selection of buffer system
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Choice of Buffer System The following criteria are used in choosing a buffer system for a particular reaction: 1. A high buffering capacity at the desired pH (i.e. choose the weak acid whose pKa is closes to the desired pH). 2. The buffer system must not affect the participants in the reaction under consideration.
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General Criteria for Biological Buffers
Buffers for biological systems should satisfy the following requirements: A pKa between 6.0 and 8.0. High water solubility and minimal organic solvent solubility. Exclusion by cellular membranes.
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