Define chromatography.explain the principle
Answers
Definition: a technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at different rates.
principle:In this technique, two phases the mobile and the stationary phase are taken in a column or burette.
The mobile phase is a liquid, gas or super critical fluid, which is forced through the stationary phase. The substance to be separated has different solubilities in the mobile phase and stationary phase.
Therefore, the components of the mixture are separated at different levels of the column as the mobile phase moves through the column.
Ex: This method is useful for the purification of elements that are present in minute qualities and impurities are not too different in their chemical properties.
Answer:
a technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at different rates. This is known as Chromatography .
Chromatography is based ona principle where molecules in a mixture applied onto the surface or into the solid and fluid stationary phase is separating from each other while moving with the aid of a mobile phase.