Question

Define precaution measures of sterilization during fermentation process

Answer 1

This chapter focuses on the concept of asepsis in bioreactors. The key process of biotechnology is fermentation and the key equipment, or the heart of the process, is the bioreactor—fermentor. The fermentation process generally employs pure culture as the biocatalyst; the success of the process depends, to a large extent, on ensuring asepsis in the fermentation system and process. Asepsis in biotechnology means freedom from unwanted microorganism(s), just as in clinical medicine, it means freedom from pathogenic microorganism(s). Asepsis in a bioreactor can be achieved through integrated efforts on system design, materials and layout, effective validation and operating procedures, scheduled checks and maintenance, and trained motivated personnel. The effectiveness of these efforts depends on the stringent adherence to schedules and procedures and on the limits of the sensitivity of sterility assessment methodology. Every effort made on the design, operation, and maintenance of a bioreactor system for minimizing opportunities of invasion by unwanted microbes is worth the trouble.

Answer 2

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Define precaution measures of sterilization during fermentation process?

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Sterilization can be achieved by a combination of heat, chemicals, irradiation, high pressure and filtration like steam under pressure, dry heat, ultraviolet radiation, gas vapor sterilants, chlorine dioxide gas etc.