define the plant tissue culture in 500 word's
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Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation.
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What is Tissue Culture?
=> A technique in which fragments of plants are cultured & grown in a laboratory.
Advantages of Tissue Culture:
- Can be grown in a short amount of time.
- Small amount of initial plant tissue required.
- Disease & viruses free plant.
- Done throughout the year.
Main steps in Tissue Culture are:-
- Selection of plant
- Isolation of explant
- Sterilization of explant
- Inoculation of explant
- Incubation
- Initiation of callus
- Sub-culturing
- Regeneration
- Hardening
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