Question

Define totel internal reflection and state its application​

Answer 1

➡️ ANSWER :-

TOTAL INTERNAL REFLECTION...

Total internal reflection refers to the complete reflection of a ray of light within a given medium from the surrounding surface. Here, the ray of light continues to be reflected within the medium (glass, water etc.) without being refracted off.

APPLICATIONS OF TOTAL INTERNAL REFLECTION ARE AS FOLLOWS...

• Selectively imaging bursts of actin activities on plasma membrane during enodcytosis
• Mapping the flow of information in cell signaling networks
• Imaging of a large number of cells simultaneously
• Study of the secretion of newly synthesized proteins
• Observation of detailed kinetics of individual exocytotic events
• Live cell imaging while minimizing photobleaching and phototoxic effects to the entire specimen

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