Question

Describe different steps involved in tissue culture technique.

Answer 1

Dear friend,

◆ Tissue culture -

Tissue culture is the growth of tissues or cells in an artificial medium separate from the organism.

● Steps in tissue culture technique -

1) Selection of mother plant

2) Initiation of culture

3) Multiplication

4) Root formation

5) Transfer to soil

# Applications of tissue culture -

1) Early flowering

2) Synthetic seeds

3) Fast multiplication

4) Genetic transformation

5) Clonal propagation

6) Disease prevention

7) Large scale multiplication

8) Self-sterlity is overcame

9) Viability of seeds improved

10) Hybridization with wide variety of plants

Hope this helped you. Thanks for asking.

Answer 2

Answer:

The initiation phase is the first phase of tissue culture. ... It is during this stage that the tissue is initiated in to culture. STAGE 2: Multiplication stage. The multiplication phase is the second step of tissue culture where the in vitro plant material is redivided and then introduced in to the medium